Fig. 2

Photomicrograph of the diaphragm of Eira barbara. (A). Presence of blood vessels (arrows) in the perimysium (1). (B). Presence of nerves inserted between the skeletal muscle fibers (arrows), where it is possible to see the epineurium (arrowhead) surrounding the nerve, in transverse (2) and longitudinal (3) sections. (C). Layer of dense connective tissue surrounding the diaphragm called the epimysium (1), invaginating and forming septa called the perimysium (arrow), from where thinner bundles of connective tissue surrounding the muscle fibers, the endomysium (arrowhead). (D). Epimysium (1), endomysium (arrowhead) and muscle fibers (arrows). HE staining. Scale bars: A, B and D 50 μm, C 100 μm