Fig. 3

Process for collecting ossification data on museum specimens. The lion embryo (MCZ: Mamm:56777) is used as an example. This specimen has been stored in 100% ethanol in the MCZ wet collections (a). The specimen undergoes micro-CT scanning in the Bruker Skyscan 1273 (b). The reconstructed output of the CT-scan consists of 2D slices containing background noise (c) and requires segmentation to create a 3D model of the developing skeleton (d). The presence or absence of each bone is scored based on the 3D model (e)