Table 1 Primer pairs designed and used for the amplification of the genes targeted in P. leptodactylus eggs
Name | Target | Sequence (5’ --> 3’) | Product length (bp) | Reference |
|---|---|---|---|---|
FGFR4.1 F FGFR4.1R | FGFR4 | 5’-ATCATAAACAAGGAGCTGAGT-3’ 5’-TCAACACCATTATASCGWGT-3’ | 130 | Present study |
FGFR4.3 F FGFR4.3R | FGFR4 | 5’-AATGTTCTAGTCAGTGARGA-3’ 5’-CTCTGGAGCCATCCAYTT-3’ | 128 | Present study |
NaKF NaKR | Na+/K+ -ATPase | 5’-GGTATGCGAAGTTCCATTT-3’ 5’-TCTCCAAGACCTCCCAGTT-3’ | 220 | [39] |
3NaK10F NaK16R | Na+/K+ -ATPase | 5’-ATGACIGTICICAYATGTGG-3’ 5’-GCRTGRTCICCIGTIACCAT-3’ | 700 | [68] |
ActF ActR | Actin | 5’-CAAGGCYGGYTTCGCYGG-3’ 5’-TCCATRTCRTCCCAGTTGG-3’ | 200 | [39] |